| 用RAPD分子标志方法研究氧化亚铁硫杆菌遗传多样性 |
| |
| 引用本文： | 贺治国,胡岳华,胡维新,钟慧,徐兢,朱敏.用RAPD分子标志方法研究氧化亚铁硫杆菌遗传多样性[J].遗传,2004,26(1):69-74. |
| |
| 作者姓名： | 贺治国 胡岳华 胡维新 钟慧 徐兢 朱敏 |
| |
| 作者单位： | 1.中南大学资源加工与生物工程学院， 长沙 410083；
2.中南大学湘雅医学院分子生物学研究中心，长沙 410078
1.School of Resource Process and Bioengineering,CSU,Changsha 410083,China;
2.Molecular Biology Research Centre,Medical School of Xiangya,CSU,Changsha 410078,China |
| |
| 摘 要： | 本文对采自7个不同环境的氧化亚铁硫杆菌进行了随机扩增多态性DNA（RAPD）分析，20个引物中筛选出扩增效果较好的4个引物，每个引物能产生1~9条DNA条带。通过4个引物的RAPD分析获得的平均相似性系数表明不同来源的菌之间的相关系数在44%~83%之间。
Abstract:Random amplified polymorphic DNA(RAPD) was used in analyzing the polymorphisms of Thiobacillus ferrooxidans from seven different places.Of the 20 primers,four could generate reproducible RAPD profiles,and each one produced 1~9 bands.The similarity coefficients obtained from profiles generated by four primers among Thiobacillus Ferrooxidans were about 44%~83%.
|
| 关 键 词： | RAPD Thiobacillus ferrooxidans 亲缘关系 Key words RAPD 氧化亚铁硫杆菌 |
| 文章编号： | 0253-9772(2004)01-0069-06 |
| 修稿时间： | 2002年11月26 |
Studies of Polymorphisms of Thiobacillus Ferrooxidans Using RAPD |
| |
| HE Zhi-Guo,HU Yue-Hua,HU Wei-Xin,ZHONG Hui,XU Jing,Zhu Min.Studies of Polymorphisms of Thiobacillus Ferrooxidans Using RAPD[J].Hereditas,2004,26(1):69-74. |
| |
| Authors: | HE Zhi-Guo HU Yue-Hua HU Wei-Xin ZHONG Hui XU Jing Zhu Min |
| |
| Institution: | School of Resource Process and Bioengineering, CSU, Changsha 410083, China. hzg13@163.com |
| |
| Abstract: | Random amplified polymorphic DNA(RAPD) was used in analyzing the polymorphisms of Thiobacillus ferrooxidans from seven different places. Of the 20 primers, four could generate reproducible RAPD profiles, and each one produced 1 approximately 9 bands. The similarity coefficients obtained from profiles generated by four primers among Thiobacillus Ferrooxidans were about 44% approximately 83%. |
| |
| Keywords: | RAPD |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《遗传》浏览原始摘要信息 |
| 点击此处可从《遗传》下载免费的PDF全文 |
|
