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Analysis of common antigen of flagella in Bacillus cereus and Bacillus thuringiensis |
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| Authors: | Tomonori Murakami Kenji Hiraoka Takeshi Mikami Tatsuji Matsumoto Susumu Katagiri Kunihiro Shinagawa Masuko Suzuki |
| Institution: | Department of Microbiology, Tohoku College of Pharmacy, Sendai, Miyagi, Japan; Quality Control Laboratory, Banyu Pharmaceutical Co. Ltd., Oosato, Saitama, Japan; Yamagata Prefectural Public Health Laboratories, Yamagata, Japan; Department of Veterinary Medicine, Faculty of Agriculture, Iwate University, Morioka, Iwate, Japan |
| Abstract: | Abstract Flagellar antigen of Bacillus cereus H.1 was purified and tested for serodiagnostic antigen by ELISA. The antibody against the flagellar antigen of B. cereus H.1 reacted not only with the homologous specific antigen but also reacted with the flagellar antigens of 23 strains of B. cereus . This common flagellar antigen of B. cereus was found to be due to 61-kDa protein by SDS-PAGE and immunoblot assay. Monoclonal antibody H15A5 against common antigenic epitope of B. cereus also reacted with flagellar antigens of 21 strains of Bacillus thuringiensis by ELISA. This monoclonal antibody reacted with the 61-kDa protein of the flagella of B. cereus H.1 and H.2 and B. thuringiensis Kurstaki HD1, Alesti and Aizawai juroi by immunoblot analysis. These results indicated that the common antigenic epitope of the 61-kDa protein existed in the flagella both of B. cereus and B. thuringiensis . |
| Keywords: | Bacillus cereus Bacillus thuringiensis Flagellar common antigen Monoclonal antibody |