Dynein arm substructure and the orientation of arm-microtubule attachments |
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Authors: | J Avolio S Lebduska P Satir |
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Institution: | Department of Anatomy Albert Einstein College of Medicine Bronx, N.Y. 10461, U.S.A. |
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Abstract: | In the presence of AMP-PCP (beta, gamma-methyleneadenosine 5'-triphosphate), a non-hydrolyzable analog of ATP, negative stain images of increased morphological detail indicate that the dynein arm, attached to ciliary doublet microtubules, is composed of subunits including a cape, an elongated body and a head. The arrangement of these subunits makes it possible to distinguish A from B subfiber binding sites on a single arm and to demonstrate that the head of an extended arm on subfiber A of one ciliary doublet is capable of binding to subfiber B of an adjacent doublet in a specific orientation, which supports a key step in a current model of the mechanochemical cycle by which the arm produces microtubule sliding in the ciliary axoneme. |
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