Fast conversion of glycerol to 1,3-propanediol by a new strain of Klebsiella pneumoniae |
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| Affiliation: | 1. Laboratory for Green Chemical Technology, Shihezi University, 832003 Xinjiang, Shihezi, PR China;2. School of Life Science and Technology, Beijing Institute of Technology, Beijing 100081, PR China;1. Key Laboratory of Tropical Marine Bio-Resources and Ecology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China;2. University of Chinese Academy of Sciences, Beijing 100049, China;1. School of Chemical and Biomolecular Engineering, Pusan National University, San 30, Jangjeon-dong, Geumjeong-gu, Busan 609-735, Republic of Korea;2. R&D Center, GS Caltex Corporation, 104-4 Munji-dong, Yusung-gu, Daejeon 305-380, Republic of Korea;1. Department of Chemical Engineering, Faculty of Engineering, University of Malaya, 50603 Kuala Lumpur, Malaysia;2. Laboratory of Chemical Engineering (Labége), BP 84234 Campus INP-ENSIACET, 4 allée Emile Monso, 31432 Toulouse Cedex 4, France;3. Laboratory of Chemical Engineering (Rangueil), University Paul Sabatier, 118 route de Narbonne, 31062 Toulouse Cedex 9, France;1. College of Forestry, Northwest A&F University, Yangling 712100, Shaanxi, China;2. CAS Key Laboratory of Science and Technology on Applied Catalysis, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, Liaoning, China;3. State Key Laboratory of Catalysis, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, Liaoning, China;4. University of Chinese Academy of Sciences, Beijing 100049, China |
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| Abstract: | Five bacterial strains screened from a batch of 39 samples could convert glycerol anaerobically to 1,3-propanediol (1,3-PD). One of the strains, XJ-Li, which could synthesize 1,3-PD with a higher concentration, was identified and characterized. Phylogenetic analysis of the strain XJ-Li included the study of morphology, physiological and biochemical characteristics. In addition, 16SrDNA sequences were created. The results indicated that this strain is a member of Klebsiella pneumoniae. The optimal cultivation parameters for pH and temperature were determined as 8.0 and 40 °C, respectively. The optimized nitrogen source and carbon source were 6.0 g/L of (NH4)2SO4 and 20 g/L of glycerol, respectively. After 8 h in batch fermentation, both the 1,3-PD concentration and glycerol consumption reached the maximum, with 12.2 g/L of 1,3-PD and 1.53 g/L h of productivity, and a molar yield of 1,3-PD to glycerol of 0.75. Fed-batch fermentation also indicated a higher molar yield of 0.70, and the concentration of 1,3-PD reached 38.1 g/L after 66.4 g/L of glycerol consumption. The results of batch and fed-batch fermentations demonstrated that K. pneumoniae XJ-Li would be an excellent 1,3-PD producer. |
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