Genetic polymorphisms and plasma levels of transforming growth factor-β1 in Chinese patients with tuberculosis in Hong Kong |
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| Institution: | 1. Division of Respiratory and Critical Care Medicine, Department of Medicine, The University of Hong Kong, Room 804, Administration Block, Queen Mary Hospital, Pokfulam Road, Hong Kong, SAR, China;2. Department of Respiratory Medicine, Kowloon Hospital, Hong Kong, SAR, China;3. Occupational and Environmental Lung Diseases Unit, Respiratory Division, Department of Medicine, The University of British Columbia, Vancouver, BC, Canada;3. Division of Nephrology and Hypertension, Department of Medicine, Vanderbilt University School of Medicine, Nashville, Tennessee 37232;4. Department of Surgery, Vanderbilt University School of Medicine, Nashville, Tennessee 37232;5. Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, Tennessee 37232;12. Department of Cell and Developmental Biology, Vanderbilt University School of Medicine, Nashville, Tennessee 37232;6. Veterans Affairs Hospital, Nashville, Tennessee 37232;1. Laboratory of Innate Immunity, CHU Ste-Justine Research Center/Department of Microbiology, Infectiology & Immunology, University of Montreal, Montreal, QC, Canada;2. Department of Biological Sciences, University of Quebec at Montreal (UQAM), Montreal, Quebec, Canada;3. Division of Hematology & Chronic Viral Illness Service, McGill University, Montreal, QC, Canada;4. CHUM/Department of Microbiology, Infectiology & Immunology, University of Montreal, Montreal, QC, Canada;5. CHU Ste-Justine Research Center/Department of Pediatrics, University of Montreal, Montreal, QC, Canada;1. Department of Ophthalmology, Far Eastern Memorial Hospital, New Taipei City, Taiwan;2. Institute of Clinical Medicine, National Taiwan University, Taipei, Taiwan;3. Department of Ophthalmology, National Taiwan University, Taipei, Taiwan;4. College of Medicine, National Taiwan University, Taipei, Taiwan;1. Department of Biochemistry, Indian Institute of Science, Bangalore, 560012, India;2. Department of Biochemistry, Central Food Technological Research Institute (CFTRI), Mysore, 570020, India;3. Department of Microbiology and Cell Biology (MCB), Indian Institute of Science, Bangalore, 560012, India |
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| Abstract: | Susceptibility to tuberculosis (TB) may be affected by host genetic factors. Elevated levels of transforming growth factor-beta 1 (TGF-β1) were found in plasma of patients with active TB compared with those of healthy contacts. To investigate the association of TGF-β1 gene polymorphisms (C-509T and T869C) and plasma levels with the risk of TB in Hong Kong Chinese adults, a case-control study was carried out on 174 active TB patients and 174 healthy controls matched for age, gender and smoking. Blood samples from 180 blood donors served as another control group. Genotyping was carried out on genomic DNA using polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP). Plasma TGF-β1 was measured by commercially available ELISA kit. We found no differences in the distribution of genotypes or alleles of TGF-β1 gene polymorphisms at C-509T and T869C between patients and either group of healthy controls. Patients with TB had elevated plasma TGF-β1 levels compared with healthy controls irrespective of their genotypes (p < 0.001). In conclusion, TGF-β1 gene polymorphism at C-509T and T869C is not associated with TB susceptibility in Hong Kong Chinese adults, but elevated plasma TGF-β1 levels suggests that this cytokine may play a role in the pathogenesis of tuberculosis. |
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