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Kinetic model for growth of Phaeodactylum tricornutum in intensive culture photobioreactor
Institution:1. Department of Life Science, Research Institute for Natural Sciences, Hanyang University, Seoul 04763, Republic of Korea;2. LMO Technology Development Team, National Marine Biodiversity Institute of Korea, Seocheon 33662, Republic of Korea;1. Institute of Plant Molecular Biology, Biology Centre CAS, Branisovska 31, 37005 Ceske Budejovice, Czech Republic;2. Faculty of Science, University of South Bohemia, Branisovska 1760, 37005 Ceske Budejovice, Czech Republic;1. Department of Civil and Environmental Engineering, University of South Florida, 4202 E Fowler Avenue, Tampa, FL 33620, USA;2. Department of Chemical, Biochemical, and Environmental Engineering, University of Maryland, Baltimore County, 1000 Hilltop Circle, Baltimore, MD 21250, USA
Abstract:A kinetic model has been developed to estimate the specific growth rate of Phaeodactylum tricornutum in batch cultures. The cultures were carried out in a laboratory scale photobioreactor. Some factors like pH, temperature and irradiance were studied. In the first case, an optimum pH of 7.8 and a specific growth rate of 0.064 h−1 were achieved for certain nitrate conditions and illumination. The temperature influence has been modelled by a modified Sinclair model. The optimum temperature was achieved at 20.4 °C in aerated cultures and at 22.3 °C in non-aerated cultures. Better adaptation to low temperatures than high ones has been obtained. The experiments carried out with different irradiances drive to a simple Monod's equation for the irradiance influence on growth, with semi-saturation irradiance of 10.2 μEinstein−2 s−1 in aerated cultures and of 6.8 μEinstein m−2 s−1 in non-aerated cultures.
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