Aspergillus fumigatus calcineurin interacts with a nucleoside diphosphate kinase |
| |
Authors: | Magnani Dinamarco Taísa Brown Neil Andrew Couto de Almeida Ricardo Sergio Alves de Castro Patrícia Savoldi Marcela de Souza Goldman Maria Helena Goldman Gustavo Henrique |
| |
Institution: | 1. Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, São Paulo, Brazil;2. Department of Microbiology, University of Londrina, Londrina, Paraná, Brazil;3. Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto, Universidade de São Paulo, São Paulo, Brazil;4. Laboratório Nacional de Ciência e Tecnologia do Bioetanol, CTBE, Caixa Postal 6170, 13083-970 Campinas, São Paulo, Brazil |
| |
Abstract: | The Ca2+-calcineurin pathway affects virulence and morphogenesis in filamentous fungi. Here, we identified 37 CalA-interacting proteins that interact with the catalytic subunit of calcineurin (CalA) in Aspergillus fumigatus, including the nucleoside diphosphate kinase (SwoH). The in vivo interaction between CalA and SwoH was validated by bimolecular fluorescence complementation. A. fumigatus swoH is an essential gene. Therefore, a temperature-sensitive conditional mutant strain with a point mutation in the active site, SwoHV83F, was constructed, which demonstrated reduced growth and increased sensitivity to elevated temperatures. The SwoHV83F mutation did not cause a loss in virulence in the Galleria mellonella infection model. Taken together these results imply that CalA interacts with SwoH. |
| |
Keywords: | |
本文献已被 ScienceDirect PubMed 等数据库收录! |
|