Molecular and histological identification of the acanthocephalan Bolbosoma cf. capitatum from the human small intestine |
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Authors: | Arizono Naoki Kuramochi Toshiaki Kagei Noboru |
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Institution: | Department of Infectious Diseases, Kyoto Prefectural University of Medicine, Kyoto, Japan; Kyoto Prefectural Institute of Public Health and Environment, Kyoto, Japan. |
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Abstract: | Acanthocephalans of the genus Bolbosoma are intestinal parasites of marine mammals with a lifecycle similar to that of anisakid nematodes. Several cases of Bolbosoma infection in humans have been reported, but no species identification has been made. Here, we report a case of Bolbosoma infection, in which the worm was found in histological sections of the partially resected small intestine of a Japanese man. Morphological features of the worm reconstructed from serial sectioning indicated that the worm was most likely to be a sexually immature female of Bolbosoma capitatum. DNA extraction from paraffin-embedded sections and ITS1-5.8S rRNA-ITS2 sequencing showed that this species formed a monophyletic group with Bolbosoma nipponicum, and was clearly distinguishable from Corynosoma spp. or Polymorphus spp. These results may provide a reference for identifying and characterizing unknown acanthocephalans found in histological sections. |
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