抗人肺癌细胞单克隆抗体的制备及其特性的研究

用人肺鳞癌细胞LTEP-78细胞系免疫Blab/c小鼠获得3株抗人肺癌细胞的单克隆抗体杂交瘤系。其中BLTI-01株经六次克隆化培养,体外传代8个月以上。BLTI-01与白细胞抗原及血型抗原基本上无交叉反应;与骨髓细胞无交叉反应;与癌胚抗原和胎甲球蛋白不相关;与肺鳞癌、肺腺癌细胞系及部分其它肿瘤细胞呈阳性反应。

Preparation of Monoclonal Antibodies Against Human Lung Cancer Cells and Study of Their Specificity

Three hybridoma lines secreting McAbs against the lung cancer cell line (LTEP-78) have been established. One of them, BLTI-01, has been subcloned six times and cultured more than eight months in vitro.The experimental data showed that the BLTI-01 has no crossreaction with human lymphocyte surface Ags (within 140 samples) , human erythrocyte surface Ags (100 samples) , marrow cell surface Ags (10 samples) , CEA and AFP. Further experiments with 24 human normal and tumour cell lines indicated that the McAb from BLTI-01 has strong positive reaction with three lung cell lines, one liver cancer, one osteoblastic sarcoma and one breast cancer lines, on the contrary, it hardly reacts with four normal tissue cell lines and the ther human caneer lines.We suggest that the corresponding Ag of McAb from BLTI-01 is a tumour associated Ag.