| Abstract: | Proliferating shoot cultures of Trifolium repens L . cv. Grasslands Huia have been established on Murashige and Skoog's medium containing 3% sucrose and 0.2 mg l−1 benzylamino purine. The shoots could be readily rooted on media without cytokinin and transferred to potting mix with 100% survival. The cultured shoots have been stored for 10 months, at 5°C in darkness without adversely affecting their potentiality for rapid multiplication. The significance of this approach for genotype storage is discussed. |
