Deleterious effects of fungizone on growth hormone and prolactin secretion by cultured GH3 cells |
| |
Authors: | C A Lapp J M Tyler M E Stachura Y S Lee |
| |
Institution: | (1) Department of Medicine, Medical College of Georgia, 30910 Augusta, Georgia;(2) VA Medical Center, 30910 Augusta, Georgia |
| |
Abstract: | Summary The rates at which growth hormone (GH) and prolactin (PRL) are spontaneously secreted from a rat pituitary tumor cell line
(GH3) were significantly reduced when these cells were maintained in medium containing 2.5 μg/ml Fungizone (Fz). The reduction
in hormone secretion was not immediately reversed by removal of Fz during perifusion, but after 3 wk in control medium, secretory
rates approached the pre-Fz treatment levels. In plated cells, secretion of GH was reduced by Fz in a dose-dependent manner,
whereas PRL secretion was significantly reduced only by the highest concentration (2.5 μg/ml) of Fz. We concluded that Fz
is not an acceptable medium constituent for the long-term culture of GH3 cells. However, because its effects are reversible, its short-term use as a decontaminating agent might eliminate the necessity
for reinitiating the culture of cells whose secretory behavior must be followed in long-term protocols.
Technical assistance provided by Y. S. Lee.
Supported by grant AM33388 to M. E. S. from the National Institutes of Health, Bethesda, MD, and in part by the Medical Research
Service of the Veterans Administration. |
| |
Keywords: | GH3 growth hormone prolactin fungizone |
本文献已被 SpringerLink 等数据库收录! |
|