Suitability of a rapid gas chromatographic method for total mesophilic bacteria and coliform enumeration in hamburgers |
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| Authors: | A. Basem,,F. Gardini,&dagger ,A. Paparella,&Dagger Maria Elisabetta, Guerzoni &dagger |
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| Affiliation: | Department of Nutrition and Food Technology, Faculty of Agriculture, University of Jordan, Amman, Jordan;†Dipartimento di Protezione e Valorizzazione Agroalimentare, Universitàdegli Studi di Bologna, Via san Giacomo 7, 40126 Bologna;‡BeCa S.p.A, Budrio, Bologna, Italy |
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| Abstract: | An instrumental method for the rapid determination of total mesophilic bacteria and total coliforms in hamburgers is described. The two procedures are based on the gas chromatographic determination of metabolic CO2 in equilibrium in the head space of sealed vials containing suitable media and inoculated with aliquots of the sample under examination. Both procedures proved to be suitable for quality control of meat products, being highly sensitive, reproducible, simple and rapid. Good correlation coefficients were obtained with plate counting for microbial concentrations ranging between 101 and 108. |
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