A fitness-based interferential genetics approach using hypertoxic/inactive gene alleles as references |
| |
Authors: | Jacques H Daniel |
| |
Institution: | (1) Centre de Génétique Moléculaire, Centre National de la Recherche Scientifique, rue de la Terrasse, 91198 Gif-sur-Yvette, France |
| |
Abstract: | Genetics, genomics, and biochemistry have all been of immense help in characterizing macromolecular cell entities and their
interactions. Still, obtaining an overall picture of the functioning of even a simple unicellular species has remained a challenging
task. One possible way to obtain a comprehensive picture has been described: by capitalizing on the observation that the overexpression
on a multicopy plasmid of apparently any wild-type gene in yeast can lead to some negative effect on cell fitness (referring
to the concept of “gene toxicity”), the FIG (fitness-based interferential genetics) approach was devised for selecting normal
genes that are in antagonistic (and potentially also agonistic) relationship with a particular gene used as a reference. Herein,
we take a complementary approach to FIG, by first selecting a “hypertoxic” allele of the reference gene—which easily provides
the general possibility of obtaining gene products with the remarkable property of being inactive without altering their macromolecular
interactivity—and then looking for the genes that interact functionally with this reference. Thus, FIG and the present approach
(Trap-FIG), both taking advantage of the negative effects on cell fitness induced by various quantitative modulations in cellular
networks, could potentially pave the way for the emergence of efficient in situ biochemistry. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|