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Analysis ofcis-regulatory elements involved in induction of a tobacco PR-5 gene by virus infection
Authors:Huguette Albrecht  Miranda D. van de Rhee  John F. Bol
Affiliation:(1) Gorlaeus Laboratories, Leiden University, Einsteinweg 5, 2333 CC Leiden, Netherlands
Abstract:cis-Regulatory elements involved in tobacco mosaic virus (TMV)-inducible expression were indentified in a tobacco PR-5 gene, encoding an acidic thaumatin-like protein. By fusing upstream sequences of the PR-5 gene to the GUS reporter gene and analysing transgenic plants containing these fusions for local and systemic induction of GUS activity by TMV, it was found that sequences between-1364 and-718 are involved in TMV induction of PR-5 gene expression.
Keywords:pathogenesis-related proteins  tobacco mosaic virus infection  cis-regulatory elements
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