Histamine modulates the cellular stress response in yeast |
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Authors: | Basil Delitheos Konstantinos Papamichael Ekaterini Tiligada |
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Institution: | (1) Department of Pharmacology, Medical School, University of Athens, M. Asias 75, 11527 Athens, Greece; |
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Abstract: | The cellular stress response is a universal protective reaction to adverse environmental or microenvironmental conditions,
such as heat and drugs, associated in part with the highly conserved heat shock proteins (HSPs). Histamine is a key inflammatory
mediator derived from l-histidine that governs vital cellular processes beyond inflammation, while recent evidence implies additional actions in
both prokaryotes and eukaryotes. This study explored the possible role of histamine in the heat shock response in yeast, an
established experimental model for the pharmacological investigation of the cellular stress response. The response was evaluated
by determining growth and viability of post-logarithmic phase grown yeast cultures after heat shock at 53°C for 30 min. Thermal
preconditioning at 37°C for 2 h served as a positive control. The effect of histamine was investigated following long-term
administration through the post-logarithmic phase of growth or short-term administration for 2 h prior to heat shock. Short-term
treatment with 1 mM histamine resulted in de novo protein synthesis-dependent acquisition of thermotolerance, while lower
doses or long-term administration of histamine failed to induce the heat-resistant phenotype. Preliminary investigation of
HSP104, HSP70 and HSP60 expression by western blotting showed an increase of these proteins after thermal preconditioning.
However, a differential HSP and tubulin expression appeared to underlie the response of yeast cells to histamine. In conclusion,
histamine was capable of inducing the adaptive phenotype, while the contribution of HSPs and tubulin and the potential implications
remain largely elusive. |
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