Black soybean extract can attenuate thrombosis through inhibition of collagen-induced platelet activation |
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Authors: | Kim Keunyoung Lim Kyung-Min Kim Chae-Wook Shin Hyun-Jung Seo Dae-Bang Lee Sang-Jun Noh Ji-Yoon Bae Ok-Nam Shin Sue Chung Jin-Ho |
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Institution: | aResearch Institute of Pharmaceutical Sciences, College of Pharmacy, Seoul National University, Seoul 151-742, South Korea;bAmore-Pacific Co. R&D Center, Gyeonggi-do 446-729, South Korea;cDepartment of Laboratory Medicine, Boramae Hospital, Seoul 156-707, South Korea |
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Abstract: | Many clinical trials have demonstrated the beneficial effects of soybean (Glycine max) on general cardiovascular health. Among a variety of soybeans, black soybean is known to display diverse biological activities superior to those of yellow and green soybeans, such as in antioxidant, anti-inflammatory and anticancer activities. However, few studies have been directed on the effect of black soybean on cardiovascular function. In this study, we aimed to investigate the effect of black soybean extract (BB) on platelet activation, a key contributor to thrombotic diseases. In freshly isolated human platelets, BB has shown potent inhibitory activity on collagen-induced platelet aggregation, while yellow soybean extract had marginal activity only. BB also attenuated serotonin secretion and P-selectin expression, which are important factors for the platelet–tissue interaction along with thromboxane A2 formation. These in vitro results were further confirmed in an ex vivo platelet aggregation measurement and in vivo venous thrombosis model where oral administration of BB reduced collagen-induced platelet aggregation and FeCl3-induced thrombus formation significantly. A potential active ingredient for antiplatelet effects of BB was isolated and identified to be adenosine through bioassay-directed fractionation and NMR and ESI-MS analyses. These results indicate that black soybean can be a novel dietary supplement for the prevention of cardiovascular risks and the improvement of blood circulation. |
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Keywords: | Abbreviations: Anti-CD62P-FITC Ab FITC-labeled anti-CD62P antibody anti-GPIb-PE Ab phycoerythrin-labeled monoclonal antibody against human glycoprotein Ib BB black soybean extract IC50 half maximal inhibitory concentration PPP platelet-poor plasma PRP platelet-rich plasma TxA2 thromboxane A2 TxB2 thromboxane B2 YB yellow soybean extract |
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