Genome-wide screen for temperature-regulated genes of the obligate intracellular bacterium, <Emphasis Type="Italic">Rickettsia typhi</Emphasis> |
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Authors: | Sheila M Dreher-Lesnick Shane M Ceraul M Sayeedur Rahman Abdu F Azad |
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Institution: | (1) Department of Microbiology and Immunology, University of Maryland, 660 W. Redwood Street, Room HH324B, 21201 Baltimore, MD, USA |
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Abstract: | Background The ability of rickettsiae to survive in multiple eukaryotic host environments provides a good model for studying pathogen-host
molecular interactions. Rickettsia typhi, the etiologic agent of murine typhus, is a strictly intracellular gram negative α-proteobacterium, which is transmitted
to humans by its arthropod vector, the oriental rat flea, Xenopsylla cheopis. Thus, R. typhi must cycle between mammalian and flea hosts, two drastically different environments. We hypothesize that temperature plays
a role in regulating host-specific gene expression, allowing R. typhi to survive in mammalian and arthropod hosts. In this study, we used Affymetrix microarrays to screen for temperature-induced
genes upon a temperature shift from 37°C to 25°C, mimicking the two different host temperatures in vitro. |
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