Crystallization,preliminary diffraction and electron paramagnetic resonance studies of a single crystal of cytochrome P450nor |
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| Affiliation: | 1. The Institute of Physical and Chemical Research (RIKEN), Hirosawa 2-1, Wako, Saitama 351-01, Japan;2. Faculty of Science, Gakushuin University, Tokyo 170, Japan;3. Division of Biological Sciences, Graduate School of Science, Hokkaido University, Sapporo 060, Japan;4. Institute of Applied Biochemistry, University of Tsukuba, Tsukuba, Ibaraki 305, Japan;5. Department of Biophysical Engineering, Faculty of Engineering Science, Osaka University, Toyonaka, Osaka 560, Japan |
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| Abstract: | Cytochrome P450nor (P450nor) is a heme-containing nitric oxide reductase from the denitrifying fungus, Fusarium oxysporum. This enzyme catalyzes the reduction of NO to N2O. In the present study, we report results from preliminary crystallographic and electron paramagnetic resonance (EPR) analysis of a single crystal of P450nor. The crystal was grown in 100 mM MES buffer at pH 5.6 using PEG 4000 as a precipitant. It belongs to the orthorhombic system with cell dimensions of a=54.99 Å, b=82.66 Å, c=87.21 Å, and the space group is P212121. The crystal diffracts synchrotron radiation at higher than 2.0 Å resolution, and therefore it is suitable for X-ray crystal structure analysis at atomic resolution. Bijvoet and dispersive anomalous difference Patterson maps show a clear peak corresponding to the heme iron. The structure solution is currently underway by means of MIR and MAD techniques. EPR analysis determined the orientation of the heme within the P450nor crystal. |
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