Identification of Myo3, a second type-II myosin heavy chain in the fission yeast Schizosaccharomyces pombe |
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| Affiliation: | 1. Division of Biology, Department of Life Sciences, Graduate School of Arts and Sciences, University of Tokyo, Komaba, Meguro-ku, Tokyo 153, Japan;2. Graduate Program in Biochemistry and Biophysics, School of Science, University of Tokyo, Hongo, Bunkyo-ku, Tokyo, Japan;3. Bionic Design Group, National Institute for Advanced Interdisciplinary Research, and Mechanical Engineering Laboratory, Tsukuba, Ibaraki, Japan |
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| Abstract: | We cloned the myo3+ gene of Schizosaccharomyces pombe which encodes a type-II myosin heavy chain. myo3 null cells showed a defect in cytokinesis under certain conditions. Overproduction of Myo3 also showed a defect in cytokinesis. Double mutant analysis indicated that Myo3 genetically interacts with Cdc8 tropomyosin and actin. Myo3 may be implicated in cytokinesis and stabilization of F-actin cables. Moreover, the function of Myo2 can be replaced by overexpressed Myo3. We observed a modest synthetic interaction between Myo2 and Myo3. Thus, Myo2 and Myo3 seem to cooperate in the formation of the F-actin ring in S. pombe. |
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