Effect of dehydroepiandrosterone sulfate on carnitine acetyl transferase activity and l-carnitine levels in oophorectomized rats |
| |
| Affiliation: | 1. Institute on Aging/Department of Medicine, University of Wisconsin-Madison, and the Wm. S. Middleton VA GRECC, Madison, WI 53706, USA;2. Metabolic Analysis Laboratories, Inc., Madison, WI 53713, USA;3. Department of Neurology, University of Wisconsin, Madison, WI 53706, USA;1. Environmental Biomonitoring Laboratory LBE (LR01/ES14), Faculty of Sciences of Bizerta, University of Carthage, Tunisia;2. Laboratory of Transmission, Control and Immunobiology of Infection (11 LR IPT 02), Pasteur Institute, Tunisia;3. Laboratory of Bioactive Substances, Biotechnology Centre in Borj-Cedria Technopol, BP.901, Hammam-lif, 2050, Tunisia;4. Laboratory of Molecular Interactions and Chemical Reactivity and Photochemical UMR CNRS 5623, University of Toulouse, University of Paul Sabatier, 118 Route de Narbonne, F-31062 Toulouse, France;1. Key Laboratory for Agro-Ecological Processes in Subtropical Region, Hunan Research Center of Livestock & Poultry Sciences, South-Central Experimental Station of Animal Nutrition and Feed Science in Ministry of Agriculture, Institute of Subtropical Agriculture, The Chinese Academy of Sciences, Changsha, Hunan 410125, PR China;2. Graduate University of Chinese Academy of Sciences, Beijing 100049, PR China;1. Department of Physics, University of Isfahan, Hezar Jarib Street, Isfahan 81746-73441, Iran;2. Institute of Biochemistry and Biophysics (IBB), University of Tehran, P.O. Box: 13145-1384, Tehran, Iran |
| |
| Abstract: | Alteration in energy metabolism of postmenopausal women might be related to the reduction of dehydroepiandrosterone sulfate (DHEAS). DHEA and DHEAS decline with age, leveling at their nadir near menopause. DHEA and DHEAS modulate fatty acid metabolism by regulating carnitine acyltransferases and CoA. The purpose of this study was to determine whether dietary supplementation with DHEAS would also increase tissue l-carnitine levels, carnitine acetyltransferase (CAT) activity and mitochondrial respiration in oophorectomized rats. Plasma l-carnitine levels rose following oophorectomy in all groups (P<0.0001). Supplementation with DHEAS was not associated with further elevation of plasma l-carnitine levels, but with increased hepatic total and free l-carnitine (P=0.021 and P<0.0001, respectively) and cardiac total l-carnitine concentrations (P=0.045). In addition, DHEAS supplementation increased both hepatic and cardiac CAT activities (P<0.0001 and P=0.05 respectively). CAT activity positively correlated with the total and free carnitine levels in both liver and heart (r=0.764, r=0.785 and r=0.700, r=0.519, respectively). Liver mitochondrial respiratory control ratio, ADP:O ratio and oxygen uptake were similar in both control and supplemented groups. These results demonstrate that in oophorectomized rats, dietary DHEAS supplementation increases the liver and heart l-carnitine levels and CAT activities. In conclusion, DHEAS may modulate l-carnitine level and CAT activity in estrogen deficient rats. The potential role of DHEAS in the regulation of fatty acid oxidation in postmenopausal women is worthy of investigation. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
