Coupling of ethanol metabolism to lipid biosynthesis: labelling of the glycerol moieties of sn-glycerol-3-phosphate,a phosphatidic acid and a phosphatidylcholine in liver of rats given [1,1-2H2]ethanol |
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| Institution: | 1. INRIA – Centre de recherche Rennes – Bretagne Atlantique, Campus de Beaulieu, Rennes F-35042, Cedex France;2. DALI–LIRMM, Université de Perpignan, 52 avenue Paul Alduy, Perpignan F-66860, France;3. Sorbonne Universités, UPMC Univ Paris 06, UMR 7606, LIP6, 4 place Jussieu, Paris F-75005, France;4. CNRS, UMR 7606, LIP6, F-75005 Paris, France;5. Sorbonne Universités, UPMC Univ Paris 06, ICS, Paris F-75005, France;1. College of Electronic and Information Engineering, South-Central University for Nationalities, Wuhan 430074, China;2. Hubei Key Laboratory of Intelligent Wireless Communication, South-Central University for Nationalities, Wuhan 430074, China;3. School of Mathematics and Computer Science, Wuhan Textile University, Wuhan 430200, China;4. School of Computer Science, South-Central University for Nationalities, Wuhan 430074, China |
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| Abstract: | The mechanism behind ethanol-induced fatty liver was investigated by administration of 1,1-2H2]ethanol to rats and analysis of intermediates in lipid biosynthesis. Phosphatidic acid and phosphatidylcholine were isolated by chromatography on a lipophilic anion exchanger and molecular species were isolated by high-performance liquid chromatography in a non-aqueous system. The glycerol moieties of palmitoyl-linoleoylphosphatidic acid, the corresponding phosphatidylcholine and free sn-glycerol-3-phosphate were analysed by GC/MS of methyl ester t-butyldimethylsilyl derivatives. The deuterium labelling in the glycerol moiety of the phosphatidic acid was 2–3-times higher than in free sn-glycerol-3-phosphate, indicating that a specific pool of sn-glycerol-3-phosphate was used for the synthesis of phosphatidic acid in liver. The results indicate that NADH formed during ethanol oxidation is used in the formation of a pool of sn-glycerol-3-phosphate that gives rise to triacylglycerol and possibly fatty liver. |
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