A Cluster of Four Novel Human G Protein-Coupled Receptor Genes Occurring in Close Proximity to CD22 Gene on Chromosome 19q13.1 |
| |
| Institution: | 1. Biomedical Research Institute Sant Pau (IIB-Sant Pau), Barcelona, Spain, C/Sant Antoni M. Claret 167, 08025 Barcelona, Spain;2. Molecular Biology and Biochemistry Department, Universitat Autònoma de Barcelona (UAB) Faculty of Medicine, Building M. Cerdanyola del Vallès, Spain;3. Biochemistry Department, Hospital de la Santa Creu i Sant Pau Barcelona, C/Sant Quintí 89, 08026 Barcelona, Spain;1. Engineering Research Center of BEEE, Ministry of Education, China, Jiangsu Provincial Key Laboratory of Solar Energy Science and Technology, School of Energy and Environment, Southeast University, Nanjing 210096, China;2. Department of Energy Science, Lund University, P.O. Box 118, SE-22100 Lund, Sweden;1. College of Pharmacy and Research Institute of Pharmaceutical Sciences, Seoul National University, Seoul 08826, Republic of Korea;2. College of Pharmacy, Dongguk University, Goyang-si, Gyeonggi-do 10326, Republic of Korea;3. Department of Pathology, College of Medicine, Chosun University, Gwangju 61452, Republic of Korea |
| |
| Abstract: | In our search for novel human galanin receptor (GALR) subtypes, human genomic DNA was PCR amplified using sets of degenerate primers based on conserved sequences in human and rat GALR. The sequence of one of the subcloned PCR products revealed homology to a sequence in the 3′ region of the human CD22 gene following a BLAST search of GenBank's database. A search for open reading frames (ORF) in the non-coding CD22 sequence resulted in identification of two novel putative intronless genes, GPR40 and GPR41. The recent submission of sequence overlapping the downstream CD22 sequence revealed a possible polymorphic insert containing a third intronless gene, GPR42, sharing 98% amino acid identity with GPR41, followed by a fourth intronless gene, GPR43. Thus, the GPR40, GPR41, GPR42, and GPR43 genes, respectively, occur downstream from CD22, a gene previously localized on chromosome 19q13.1. The four putative novel human genes encode new members of the GPCR family and share little homology with GALR. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
