卫星搭载亚麻后代中PEG和NaCl

把空间生物学和细胞工程相结合，通过组织培养技术对其离体筛选，得到抗1.2% NaCl和35% PEG的愈伤组织。将所得抗性系愈伤组织在2.0 mg/L 6-苄基氨基嘌呤、0.5 mg/L吲哚乙酸的MS培养基上分化得到完整的植株。抗性系能在胁迫条件下保持高的生长速度和高效的脯氨酸合成能力。表明空间诱变与组织培养相结合有望可成为筛选抗胁迫变异系的有效途径。

In vitro selection of salt and PEG tolerant flaxi lines from the progeny of flown seeds

Based on the effects of spaceflight on the seeds,the salt and PEG tolerant calluses were obtained from the second post spaceflight generation seedlings by selection on MS medium containing 1.2% NaCl or 35% PEG.The plants regenerated from the selected calluses on MS medium with 2.0 mg/L 6 benzyladenine and 0.5 mg/L indole 3 acetic acid.The selected tolerant calluses shown a higher tolerance to osmotic stress than the nonselected callus derived from the second generation seedlings of uncarried flax seeds.The selected calluses appeared a higher efficiency to accumulate proline.It was indicated that the combination of space mutagenesis with tissue culture can be used for the selection of stress tolerant flax lines.