Genetic organization and conjugal plasmid DNA transfer of pHP69, a plasmid from a Korean isolate of Helicobacter pylori |
| |
Authors: | Jung-Soo Joo Jae-Young Song Seung-Chul Baik Woo-Kon Lee Myung-Je Cho Kon-Ho Lee Hee-Shang Youn Ji-Hyun Seo Kwang-Ho Rhee Hyung-Lyun Kang |
| |
Affiliation: | 4. Laboratory of Biochemistry and Genetics, National Institute of Diabetes, Digestive, and Kidney Diseases, National Institutes of Health, Bethesda, MD, 20892, USA 1. Department of Microbiology, Gyeongsang National University School of Medicine, Jinju, 660-751, Republic of Korea 3. Research Institute of Life Science, Gyeongsang National University, Jinju, 660-701, Republic of Korea 2. Department of Pediatrics, Gyeongsang National University School of Medicine, Jinju, 660-751, Republic of Korea
|
| |
Abstract: | We isolated pHP69, a 9,153 bp plasmid from Helicobacter pylori with a 33.98% (G+C) content. We identified 11 open reading frames (ORFs), including replication initiation protein A (repA), fic (cAMP-induced filamentation protein), mccC, mccB, mobA, mobD, mobB, and mobC, as well as four 22 bp tandem repeat sequences. The nucleic acid and predicted amino acid sequences of these ORFs exhibited significant homology to those of other H. pylori plasmids. pHP69 repA encodes a replication initiation protein and its amino acid sequence is similar to those of replicase proteins from theta-type plasmids. pHP69 contains two types of repeat sequences (R1 and R2), a MOBHEN family mobilization region comprising mobC, mobA, mobB, and mobD, and genes encoding microcin B and C. Among the 36 H. pylori strains containing plasmids, mobA or mccBC are present in 12 or 6, respectively and 3 contain both genes. To examine intrinsic capability of H. pylori for conjugative plasmid transfer, a shuttle vector pBHP69KH containing pHP69 and replication origin of pBR322 was constructed. It was shown that this vector could stably replicate and be mobilized among clinical H. pylori strains and demonstrated to gene transfer by natural plasmid. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|