Production of trans-10, cis-12 Conjugated Linoleic Acid in Rice |
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Authors: | Junko Kohno-Murase Mari Iwabuchi Saori Endo-Kasahara Koichi Sugita Hiroyasu Ebinuma Jun Imamura |
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Institution: | (1) Plantech Research Institute, 1000 kamoshida-cho, Aoba-ku, 227-0033 Yokohama, Japan;(2) Forestry Science Laboratory, Nippon Paper Industries CO., LTD., 5-21-1, Oji, Kita-ku, 114-0002 Tokyo, Japan;(3) Present address: Graduate school of Bioscience and Biotechnology, Tokyo Institute of Technology, 4259 Nagatsuta-cho, Midori-ku 226-8501, Yokohama, Japan;(4) Present address: Faculty of Agriculture, Tamagawa University, 6-1-1, Tamagawa-gakuen, Machida 194-8610, Tokyo, Japan |
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Abstract: | Conjugated linoleic acid (CLA) has anti-carcinogenic and anti-atherosclerosis activity, and modulatory effects on the immune
system and lipid metabolism. To produce a transgenic rice plant that can accumulate CLA, a linoleate isomerase gene that can
convert linoleic acid to trans-10, cis-12 CLA was introduced and expressed under the control of seed-specific promoters from the oleosin and globulin genes. The
fatty acid composition of the transgenic rice grain was analyzed by gas chromatography. Although there was no clear difference
in the fatty acid composition between seeds from transformed versus untransformed plants, a peak of trans-10, cis-12 CLA methyl ester, which was not present in seeds from untransformed plants, was found in transformed plants. The trans-10, cis-12 CLA comprised an average of 1.3% (w/w) of the total fatty acids in seeds carrying the oleosin promoter in comparison to
0.01% (w/w) in seeds carrying the globulin promoter. In addition, approximately 70 and 28% of the total amount of the CLA
isomer were present in the triacylglycerol and free fatty acid fractions, respectively. These results demonstrate the ability
to produce fatty acid components of vegetable oils with novel physiological activities in crops. |
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Keywords: | CLA conjugated linoleic acid linoleate isomerase MAT vector transgenic rice |
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