Selenite-stress selected mutant strains of probiotic bacteria for Se source production |
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| Affiliation: | 1. Central Laboratory, Faculty of Agricultural and Food Sciences and Environmental Management, University of Debrecen, Böszörményi út 138, Debrecen H-4032, Hungary;2. Department of Biotechnology and Microbiology, Faculty of Science and Technology, University of Debrecen, Egyetem tér 1, Debrecen H-4032, Hungary;3. Institute of Bio- and Environmental Energetics, University of Debrecen, Egyetem tér 1, Debrecen H-4032, Hungary;1. Environmental Engineering and Water Technology Department, UNESCO-IHE Institute for Water Education, PO Box 3015, Delft DA 2601, The Netherlands;2. Biofouling and Biofilm Processes Section of Water and Steam Chemistry Division, Bhabha Atomic Research Centre, Kalpakkam, 603102, Tamil Nadu, India;3. Department of Chemistry and Bioengineering, Tampere University of Technology, PO Box 541, Tampere, Finland;1. Dairy Microbiology Division, National Dairy Research Institute, Karnal, Haryana 132001, India;2. Department of Dairy Microbiology, College of Dairy Technology, Chhattisgarh Kamdhenu Vishwavidyalya, Raipur, Chhattisgarh 492006, India;3. Department of Food Science, University of Missouri, USA;4. Directorate of Knowledge Management in Agriculture, Indian Council of Agricultural Research, New Delhi, India;1. Key Laboratory of Dairy Science, Ministry of Education, Department of Food Science, Northeast Agricultural University, Harbin 150030, China;2. National Research Centre of Dairy Engineering and Technology, Northeast Agricultural University, Harbin 150028, China;3. Synergetic Innovation Centre of Food Safety and Nutrition, Harbin 150030, China;1. State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan, 430070, PR China;2. Institute of Environmental Microbiology, College of Resources and Environment, Fujian Agriculture & Forestry University, Fuzhou, Fujian 350002, PR China;1. Department of Biotechnology, University of Verona, Strada Le Grazie 15, 37134 Verona, Italy;2. Department of Computer Science, University of Verona, Strada Le Grazie 15, 37134 Verona, Italy;3. Biosciences, College of Life and Environmental Sciences, University of Exeter, Stocker Road, Exeter EX4 4QD, UK;4. Biofilm Research Group, Department of Biological Sciences, University of Calgary, Calgary, Alberta, Canada;2. Programa de Pós-Graduação em Química, Universidade Federal de Pelotas (UFPel), Campus Universitário, S/N, CEP 96160-000, Capão do Leão, RS, Brazil |
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| Abstract: | Selenium deficiency is a major health problem worldwide for about 1 billion people. Bacterial cells usually possess low tolerance to selenite stress and also low ability to reduce high concentrations of toxic selenite. Here, high tolerance to selenite and selenium bioaccumulation capability were developed in mutated clones of probiotic and starter bacteria including Enterococcus faecium, Bifidobacterium animalis ssp. lactis, Lactobacillus casei and Lactococcus lactis ssp. lactis by food-level strain development process and clone selection. All mutant clones possessed increased glutathione concentration and glutathione reductase activity. The selenite treatment increased further these values in L. casei mutant strain pointing at a different selenite reduction pathway and/or stress response in this organism. Considerable conversion of selenite to cell bound selenium forms with a concomitant high biomass production was detected in E. faecium and B. animalis ssp. lactis cultures. Possible application of these strains as food and feed supplements is under investigation. |
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| Keywords: | Selenite Selenium Lactic acid bacteria Glutathione Glutathione reductase DTNB" },{" #name" :" keyword" ," $" :{" id" :" kw0035" }," $$" :[{" #name" :" text" ," _" :" 5,5′-dithobis-(2-nitrobenzoic acid) GR" },{" #name" :" keyword" ," $" :{" id" :" kw0045" }," $$" :[{" #name" :" text" ," _" :" glutathione reductase GSH" },{" #name" :" keyword" ," $" :{" id" :" kw0055" }," $$" :[{" #name" :" text" ," _" :" glutathione TEA" },{" #name" :" keyword" ," $" :{" id" :" kw0065" }," $$" :[{" #name" :" text" ," _" :" triethanolamine |
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