Synergistic effect of Se-methylselenocysteine and vitamin E in ameliorating the acute ethanol-induced oxidative damage in rat |
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| Institution: | 1. State Key Lab of Food Science and Technology, Nanchang University, Nanchang 330047, China;2. Nanoscale Science and Technology Laboratory, Institute for Advanced Study, Nanchang University, Nanchang 330031, China;3. Chuanqi Pharmaceutical Corp. Ltd, Jiangxi Povince, Nanchang 330047, China;1. College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, PR China;2. Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, PR China;3. Bijie Pilot Area Research Institute, Bijie University, Bijie 551700, PR China;1. Department of Epidemiology, Graduate School of Public Health, University of Pittsburgh, PA, USA;2. Department of Nursing, College of Nursing, Korea University, Seoul, Republic of Korea;3. Department of Research, Kuakini Medical Center, and Department of Geriatric Medicine, John A. Burns School of Medicine, University of Hawaii, Honolulu, USA;4. Department of Public Health, Shiga University of Medical Science, Seta Tsukinowa-cho, Otsu, Shiga, Japan;5. Department of Internal Medicine, University of Pittsburgh Medical Center, Pittsburgh, PA, USA;6. Center for Epidemiologic Research in Asia, Shiga University of Medical Science, Seta Tsukinowa-cho, Otsu, Shiga, Japan;7. Sleep and Critical Care Medicine, Department of Internal Medicine, Korea University Ansan Hospital, Ansan, Republic of Korea |
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| Abstract: | The present study was conduced to investigate the synergistic effects of combined treatments with Se-methylselenocysteine (SeMSC) and vitamin E (Vit E) in reversing oxidative stress induced by ethanol in serum and different tissues of rats. Sixty female rats were randomly divided into six groups for 30 days’ consecutive pretreatments as followed: control (I), physiological saline (II), 2.8 μg kg?1 Se as SeMSC (III), 2.8 μg kg?1 Se as sodium selenite (Na2SeO3, IV), 5 mg kg?1 α-tocopherol as α-tocopherol acetate (Vit E, V), 5 mg kg?1 α-tocopherol as α-tocopherol acetate and 2.8 μg kg?1 Se as SeMSC (VI). All animals in groups II–VI were treated by ethanol treatment to cause oxidative stress. After 6 h of ethanol treatment, the activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), the contents of total antioxidant capacity (T-AOC), malondialdehyde (MDA), glutathione (GSH) and carbonyl protein (CP) in the serum, liver, heart and kidney were measured. The result showed that the individual SeSMC, Na2SeO3 and vitamin E could effectively increase the SOD, T-AOC, GSH-Px and GSH contents as well as significantly decrease the MDA and CP concentrations in the tissues of ethanol-induced rats. At the same dose on different forms of Se, SeMSC showed greater antioxidant activity than Na2SeO3. Moreover, group VI (SeMSC and α-tocopherol acetate) showed much better antioxidant activity than individual group III (SeMSC) and V (α-tocopherol acetate) due to the synergistic effect. |
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| Keywords: | Se-methylselenocysteine Synergistic effect Vitamin E Antioxidant activity |
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