Biomedical potential of actinobacterially synthesized selenium nanoparticles with special reference to anti-biofilm,anti-oxidant,wound healing,cytotoxic and anti-viral activities |
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| Institution: | 1. Actinobacterial Research Laboratory, Department of Microbiology, School of Biosciences, Periyar University, Periyar Palkalai Nagar, Salem 636011, Tamil Nadu, India;2. Center for Nanoscience and Nanotechnology, Periyar University, Periyar Palkalai Nagar, Salem 636011, Tamil Nadu, India;1. Glyn O. Phillips Hydrocolloid Research Centre at HUT, School of Food and Pharmaceutical Engineering, Faculty of Light Industry, Hubei University of Technology, Wuhan 430068, China;2. Department of Chemistry, Glyndwr University, Plas Coch, Mold Road, Wrexham LL11 2AW, UK;3. Glyn O. Phillips Hydrocolloid Research Center, Glyndwr University, Plas Coch, Mold Road, Wrexham LL11 2AW, UK;1. Department of Inorganic Chemistry, Faculty of Chemical Technology, University of Chemistry and Technology, Prague, Technická 5, 166 28 Prague 6, Czech Republic;2. Department of Food Analysis and Nutrition, Faculty of Food and Biochemical Technology, University of Chemistry and Technology, Prague, Technická 5, 166 28 Prague 6, Czech Republic;3. Department of Biochemistry and Microbiology, Faculty of Food and Biochemical Technology, University of Chemistry and Technology, Prague, Technická 3, 166 28 Prague 6, Czech Republic;4. Department of Solid State Engineering, Faculty of Chemical Technology, University of Chemistry and Technology, Prague, Technická 3, 166 28 Prague 6, Czech Republic;1. Pharmaceutics Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, Iran;2. Herbal and Traditional Medicines Research Center, Kerman University of Medical Sciences, Kerman, Iran;3. Department of Microbiology and Immunology, Kerman University of Medical Sciences, Kerman, Iran;4. Research Center for Tropical and Infectious Diseases, Kerman University of Medical Sciences, Kerman, Iran;5. Environmental Health Engineering Research Centers, Kerman University of Medical Sciences, Kerman, Iran |
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| Abstract: | Currently, there is an ever-increasing need to develop environmentally benign processes in place of synthetic protocols. As a result, researchers in the field of nanoparticle synthesis are focusing their attention on microbes from rare biological ecosystems. One potential actinobacterium, Streptomyces minutiscleroticus M10A62 isolated from a magnesite mine had the ability to synthesize selenium nanoparticles (SeNPs), extracellularly. Actinobacteria mediated SeNP synthesis were characterized by UV–visible, Fourier transform infrared (FT-IR), X-ray diffraction (XRD), energy dispersive X-ray spectroscopy (EDX) and high resolution transmission electron microscopy (HR-TEM) analysis. The UV-spectral analysis of SeNPs indicated the maximum absorption at 510 nm, FT-IR spectral analysis confirms the presence of capping protein, peptide, amine and amide groups. The selenium signals confirm the presence of SeNPs. All the diffraction peaks in the XRD pattern and HR-TEM confirm the size of SeNPs in the range of 10–250 nm. Further, the anti-biofilm and antioxidant activity of the SeNPs increased proportionally with rise in concentration, and the test strains reduced to 75% at concentration of 3.2 μg. Selenium showed significant anti-proliferative activity against HeLa and HepG2 cell lines. The wound healing activity of SeNPs reveals that 5% selenium oinment heals the excision wound of Wistar rats up to 85% within 18 days compared to the standard ointment. The biosynthesized SeNPs exhibited good antiviral activity against Dengue virus. The present study concludes that extremophilic actinobacterial strain was a novel source for SeNPs with versatile biomedical applications and larger studies are needed to quantify these observed effects of SeNPs. |
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| Keywords: | Selenium nanoparticle Anti-biofilm Wound healing Anti-proliferative |
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