Development of a novel purification method for AAV vectors using tangential flow filtration |
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| Authors: | Rimi Miyaoka Yuji Tsunekawa Yae Kurosawa Takako Sasaki Azusa Onodera Kenji Sakamoto Yuko Kakiuchi Mikako Wada Yuko Nitahara-Kasahara Hiromi Hayashita-Kinoh Takashi Okada |
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| Affiliation: | 1. Division of Molecular and Medical Genetics, Center for Gene and Cell Therapy, The Institute of Medical Science, The University of Tokyo, Tokyo, Japan;2. Division of Molecular and Medical Genetics, Center for Gene and Cell Therapy, The Institute of Medical Science, The University of Tokyo, Tokyo, Japan Chromatography Media Business Division, HOYA Technosurgical Corporation, Tokyo, Japan |
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| Abstract: | Adeno-associated virus (AAV) vector can efficiently transduce therapeutic genes in various tissue types with less side effects; however, owing to complex multistep processes during manufacture, there have been surges in the pricing of recently approved AAV vector-based gene therapy products. This study aimed to develop a simple and efficient method for high-quality purification of AAV vector via tangential flow filtration (TFF), which is commonly used for concentration and diafiltration of solutions during AAV vector purification. We established a novel purification method using TFF and surfactants. Treatment with two classes of surfactants (anionic and zwitterionic) successfully inhibited the aggregation of residual proteins separated from the AAV vector in the crude product by TFF, obtaining a clearance of 99.5% residual proteins. Infectivity of the AAV vector purified using the new method was confirmed both in vitro and in vivo, and no remarkable inflammation or tissue damage was observed in mouse skeletal muscle after local administration. Overall, our proposed method could be used to establish a platform for the purification of AAV vector. |
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| Keywords: | adeno-associated virus purification surfactant tangential flow filtration ultrafiltration |
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