The ATP pool in relation to the production of glycerol and heat during growth of the halotolerant yeast Debaryomyces hansenii |
| |
Authors: | Lena Gustafsson |
| |
Institution: | (1) Department of Marine Microbiology, Botanical Institute, University of Göteborg, Carl Skottsbergs Gata 22, S-413 19 Göteborg, Sweden |
| |
Abstract: | In a study of the halotolerant yeast Debarymyces hansenii cultured in 4 mM and 2.7 M NaCl the intracellular ATP pool, the heat production, the oxygen uptake, and, in the high culture salinity also, the intracellular glycerol concentration were found to be correlated. The intracellular ATP in the 2.7 M NaCl culture had a constant concentration of 3.5 mM ATP during the second half of the lag phase, while in 4 mM NaCl it rose to a maximum of 3.1 mM during the late log phase. The intracellular glycerol concentration in 2.7 M NaCl was about 1.3M during the entire exponential growth phase. Sine the glycerol concentration of the medium was not more than 0.23 mM, glycerol must contribute to the osmotic balance of the cells in high salinity. The corresponding maximum values for the 4 mM NaCl culture were 0.16 M and 0.08 mM. The experimental enthalpy changes were approximately the same for the two salinities, viz. about-1200 kJ per mole consumed glucose. The Y
m-values for the 4 mM and 2.7 M NaCl cultures were 91 and 59, respectively, the difference being a consequence of the decreased efficiency of growth in high salinity.Abbreviations CFU
colony-forming units
- PCA
perchloric acid
- TCA
trichloroacetic acid |
| |
Keywords: | Halotolerant yeast Debaryomyces hansenii ATP pool Glycerol production Microcalorimetry |
本文献已被 SpringerLink 等数据库收录! |
|