The effect of combination treatment with docosahexaenoic acid and 5-fluorouracil on the mRNA expression of apoptosis-related genes, including the novel gene BCL2L12, in gastric cancer cells |
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Authors: | Zhihong Zhuo Leming Zhang Qitian Mu Yanru Lou Zhaohui Gong Yijiu Shi Guifang Ouyang Yi Zhang |
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Affiliation: | 1. School of Medicine, Ningbo University, 818 Fenghua Road, Ningbo, 315211, Zhejiang Province, People’s Republic of China 2. Ningbo No. 1 Hospital, Ningbo, 315010, Zhejiang Province, People’s Republic of China
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Abstract: | Docosahexaenoic acid (DHA) plays an important role in suppressing the growth of cancer. In this paper, the synergetic anticancer effect of combination DHA with 5-fluorouracil (5-FU) was investigated in gastric carcinoma cells. We found that DHA inhibited the growth of cultured SGC7901 cells at different concentrations in a dose- and time-dependent manner. Furthermore, the growth-inhibition activities of increasing concentration of 5-FU were markedly enhanced when different doses of 5-FU were administered in the combination with dose as low as 40 μg/ml of DHA. The early phase of apoptosis was increased in DHA- and 5-FU-treated cells. In the case of apoptotic genes expression in the combination-treated cells, BAX mRNA expression increased, whereas FAS, BCL-2, BCL2L12, and CASPASE-9 mRNA expression decreased. These results suggest that DHA strongly enhances the anticancer effect of 5-FU. Moreover, the application of both compounds on gastric cancer cells provides a new potential approach for cancer therapy. |
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