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Propagation in vitro of the apple rootstock M4: effect of phytohormones on shoot quality
Authors:David I Dunstan  Keith E Turner  Wayne R Lazaroff
Institution:(1) Plant Tissue Culture Research Group, Kelowna Nurseries Ltd., P.O. Box 178, V1Y 7N5 Kelowna, British Columbia, Canada
Abstract:The quality of shoots in cultures of the apple rootstock, M4, was used as a criterion for the selection of an optimum medium. The frequency of shoots in defined shoot clases was monitored for each of five media, which differed in the type and concentration of phytohormone. Media containing BA (1.15 mg l-1) and IBA (either 0.15 or 0.20 mg l-1) produced the maximum number of shoots that were desirable for transplantation and acclimatization.
Keywords:Shoot population  apple shoot cultures  shoot quality  micropropagation
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