Concurrent genotyping of <Emphasis Type="Italic">Helicobacter pylori</Emphasis>virulence genes and human cytokine SNP sites using whole genome amplified DNA derived from minute amounts of gastric biopsy specimen DNA |
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| Authors: | Anna Ryberg Kurt Borch Yi-Qian Sun Hans-Jürg Monstein |
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| Institution: | 1.Divisions of Surgery,University Hospital,85 Link?ping,Sweden;2.Clinical Microbiology,University Hospital,85 Link?ping,Sweden;3.Department of Clinical and Experimental Medical, Faculty of Health Sciences,Link?ping University,85 Link?ping,Sweden |
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| Abstract: | Background Bacterial and cellular genotyping is becoming increasingly important in the diagnosis of infectious diseases. However, difficulties
in obtaining sufficient amount of bacterial and cellular DNA extracted from the same human biopsy specimens is often a limiting
factor. In this study, total DNA (host and bacterial DNA) was isolated from minute amounts of gastric biopsy specimens and
amplified by means of whole genome amplification using the multiple displacement amplification (MDA) technique. Subsequently,
MDA-DNA was used for concurrent Helicobacter pylori and human host cellular DNA genotyping analysis using PCR-based methods. |
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