Validation of internal control for gene expression study in soybean by quantitative real-time PCR |
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| Authors: | Bo Jian Bin Liu Yurong Bi Wensheng Hou Cunxiang Wu Tianfu Han |
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| Institution: | (1) School of Life Sciences, Lanzhou University, Lanzhou, Gansu, 730000, PR China;(2) The National Key Facility for Crop Gene Resources and Genetic Improvement (NFCRI), Institute of Crop Sciences, The Chinese Academy of Agricultural Sciences, Beijing, 100081, PR China |
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| Abstract: | Background Normalizing to housekeeping gene (HKG) can make results from quantitative real-time PCR (qRT-PCR) more reliable. Recent studies
have shown that no single HKG is universal for all experiments. Thus, a suitable HKG should be selected before its use. Only
a few studies on HKGs have been done in plants, and none in soybean, an economically important crop. Therefore, the present
study was conducted to identify suitable HKG(s) for normalization of gene expression in soybean. |
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