Insulin stimulates generation of intracellular mediators in rat heart |
| |
| Authors: | S L Macaulay J O Macaulay L Jarett |
| |
| Institution: | 1. Department of Cell Physiology and Metabolism, University of Geneva Medical Center, rue Michel-Servet 1, 1206 Geneva, Switzerland;1. Department of Chemistry, Saint Louis University, Saint Louis, MO 63103, USA;2. Department of Chemistry and Biochemistry, School of Green Chemistry and Engineering, The University of Toledo, Toledo, OH 43606, USA |
| |
| Abstract: | Insulin treatment of rats results in an increased amount or activity of insulin mediators in heart muscle. The mediators stimulated mitochondrial pyruvate dehydrogenase and inhibited glucagon-stimulated adenylate cyclase. The mediators were copurified by ultrafiltration, ethanol extraction, Dowex cation-exchange, and QAE-Sephadex anion-exchange chromatography. The activities of the two mediators were separated by Sephadex G-10 chromatography. Fasting rats for 72 h diminished the mediator response to insulin treatment. These results, taken together with previous reports, indicate that insulin generates a number of mediators which have a ubiquitous tissue distribution. The activity of these mediators, like insulin responsiveness, is altered by the metabolic state of the animal. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
