Analysis of absorption spectra of purple bacterial reaction centers in the near infrared region by higher order derivative spectroscopy |
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Authors: | Mikhailyuk I K Knox P P Paschenko V Z Razjivin A P Lokstein H |
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Affiliation: | A.N. Belozerski Institute of Physico-Chemical Biology, Biology Faculty of the M.V. Lomonosov Moscow State University, 119992, Moscow, Russia. |
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Abstract: | Reaction centers (RCs) of purple bacteria are uniquely suited objects to study the mechanisms of the photosynthetic conversion of light energy into chemical energy. A recently introduced method of higher order derivative spectroscopy [I.K. Mikhailyuk, H. Lokstein, A.P. Razjivin, A method of spectral subband decomposition by simultaneous fitting the initial spectrum and a set of its derivatives, J. Biochem. Biophys. Methods 63 (2005) 10-23] was used to analyze the NIR absorption spectra of RC preparations from Rhodobacter (R.) sphaeroides strain 2R and Blastochloris (B.) viridis strain KH, containing bacteriochlorophyll (BChl) a and b, respectively. Q(y) bands of individual RC porphyrin components (BChls and bacteriopheophytins, BPheo) were identified. The results indicate that the upper exciton level P(y+) of the photo-active BChl dimer in RCs of R. sphaeroides has an absorption maximum of 810nm. The blue shift of a complex integral band at approximately 800nm upon oxidation of the RC is caused primarily by bleaching of P(y+), rather than by an electrochromic shift of the absorption band(s) of the monomeric BChls. Likewise, the disappearance of a band peaking at 842nm upon oxidation of RCs from B. viridis indicates that this band has to be assigned to P(y+). A blue shift of an absorption band at approximately 830nm upon oxidation of RCs of B. viridis is also essentially caused by the disappearance of P(y+), rather than by an electrochromic shift of the absorption bands of monomeric BChls. Absorption maxima of the monomeric BChls, B(B) and B(A) are at 802 and 797nm, respectively, in RCs of R. sphaeroides at room temperature. BPheo co-factors H(B) and H(A) peak at 748 and 758nm, respectively, at room temperature. For B. viridis RCs the spectral positions of H(B) and H(A) were found to be 796 and 816nm, respectively, at room temperature. |
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Keywords: | BChl, bacteriochlorophyll B, monomeric accessory BChls BPheo or H, bacteriopheophytin QA, primary quinone acceptor EET, excitation energy transfer FWHM, full width at half maximum LDAO, lauryldimethylamine-oxide NIR, near infrared P, primary electron donor Py+ and Py−, symmetric and antisymmetric components of the Qy transition of P RC, reaction center RT, room temperature B., Blastochloris R., Rhodobacter |
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