Clonal plant production from self- and cross-pollinated seed families of <Emphasis Type="Italic">Pinus sylvestris</Emphasis> (L.) through somatic embryogenesis |
| |
Authors: | Marie-Anne Lelu-Walter Michèle Bernier-Cardou Krystyna Klimaszewska |
| |
Institution: | (1) INRA, Unité Amélioration, Génétique et Physiologie Forestières, Centre Recherches d’Orléans, BP 20 619, Ardon, 45166 Olivet Cedex, France;(2) Natural Resources Canada, Canadian Forest Service, Laurentian Forestry Centre, 1055 du P.E.P.S., P.O. Box 10380, Stn. Sainte-Foy, G1V 4C7 Quebec, Quebec, Canada |
| |
Abstract: | Several factors affecting somatic embryogenesis (SE) in Pinus sylvestris from self- and cross-pollinated seed families were studied with the aim of producing large quantities of clonal plants. Somatic
embryogenesis initiation from zygotic embryos was improved on a medium with lower than standard concentrations of 2,4-dichlorophenoxyacetic
acid (2.2 vs. 9.5 μM) and 6-benzyladenine (2.2 vs. 4.5 μM). On this medium, initiation rates of four controlled crosses, including
one self-cross, varied from 3% to 25%. Among the maturation factors tested, the concentration of abscisic acid (ABA 80, 120 μM)
had no significant effect on the production of mature somatic embryos when the medium contained 0.1 M sucrose. When sucrose
concentration was 0.2 M, however, 1.4 times more mature somatic embryos were produced on medium with 80 μM compared with 120 μM
ABA. Under our best maturation conditions, mature somatic embryos accumulated amounts of storage proteins that were similar
to the amounts in mature zygotic embryos. Activated charcoal exerted a beneficial effect on mature somatic embryo production
of 24-week-old cultures; there was no evidence of such an effect in 8-week-old cultures. Thirty-seven embryogenic lines from
a self-cross and an out-cross were chosen for clonal plant production. Highly embryogenic lines produced mature somatic embryos
that were more likely to convert to plants than those from less embryogenic lines. After 4 months of growth in a shade house,
plantlet survival rates exceeded 70% for 31 lines out of 35. This report describes an improved method for accelerated production
of large quantities of Scots pine for clonal tests. |
| |
Keywords: | Maturation Scots pine Somatic embryos Storage proteins |
本文献已被 SpringerLink 等数据库收录! |
|