| 糖尿病肝脏基因表达谱代谢学分析 |
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| 引用本文： | 赵晓龙,王宣春,鹿斌,杨叶红,胡仁明.糖尿病肝脏基因表达谱代谢学分析[J].现代生物医学进展,2009,9(19). |
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| 作者姓名： | 赵晓龙 王宣春 鹿斌 杨叶红 胡仁明 |
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| 作者单位： | 复旦大学附属华山医院内分泌科,上海,200040 |
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| 基金项目： | ,国家自然科学基金重点项目,上海市科学技术委员会重大项目,国家自然科学基金 |
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| 摘 要： | 目的:探讨糖尿病患者肝脏与正常对照肝脏基因表达谱的变化,并进行代谢通路生物信息学分析.方法:应用Affymetrix的Human Genome U133A array芯片检测糖尿病肝脏组织(n=2)和正常对照肝脏组织(n=2)的基因表达谱变化,通过DAVID分析平台对芯片监测到的表达上调的基因进行KEGG通路分析,并用RT-PCR验证部分基因的表达情况.结果:以比值大于2或小于-2为准,共有492条基因明显上调,820条基因明显下调;在差异表达明显的前20位基因中,涉及氧化应激、免疫炎症反应和脂代谢.KEC-G代谢通路分析显示:该差异表达基因谱符合2型糖尿病的发病机制,其差异表达明显基因的代谢通路涉及胰岛素信号通路、脂代谢、补体和凝血系统、糖代谢、粘附功能和细胞因子和受体的相互作用.RT-PCR证实差异表达明显基因SOD2mRNA和CRPmRNA确实表达明显上调.结论:糖尿病肝脏与正常肝脏组织基因表达谱的变化在代谢通路上主要表现为在糖、脂代谢和胰岛素信号通路、补体和凝血系统等的改变,肝脏在糖尿病发病机制中的作用可能与其参与糖脂代谢和胰岛素的作用异常有关.
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| 关 键 词： | 糖尿病 基因芯片 KEGG 代谢通路分析 |
Metabolic pathway analysis on gene profiling of diabetic liver |
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| ZHAO Xiao-long,WANG Xuan-chun,LU Bin,YANG Ye-hong,HU Ren-ming.Metabolic pathway analysis on gene profiling of diabetic liver[J].Progress in Modern Biomedicine,2009,9(19). |
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| Authors: | ZHAO Xiao-long WANG Xuan-chun LU Bin YANG Ye-hong HU Ren-ming |
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| Institution: | ZHAO Xiao-long,WANG Xuan-chun,LU Bin,YANG Ye-hong,HU Ren-ming(Department of Endocrinology,Huashan Hospital,Fudan University,Shanghai 200040) |
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| Abstract: | Objective:To investigate gene profile in diabetic liver tissue and perform metabolic pathway analysis among significantly increased or decreased expression gene.Methods: OLigonucleotide array was utilized to screen the defference of gene expression in both diabetic liver and normal liver tissue.KEGG metabolic pathway was performed among significantly increased or decreased gene.RT-PCR was performed to assessthe mRNA level of the gene.Results: Compare with the matched normal tissue,492 genes was upregulated ... |
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| Keywords: | diabetes mellitus gene chip KEGG metabolism pathway analysis |
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