Thermostabilization of protein A-luciferase fusion protein by single amino acid mutation |
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| Authors: | Takashi Ebihara Hiromasa Takayama Yasuko Yanagida Eiry Kobatake Masuo Aizawa |
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| Institution: | (1) Department of Biological Information, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Nagatsuta, Midori-ku, Yokohama, 226-8501, Japan |
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| Abstract: | A gene expression plasmid, pMALU7, for coding a fusion protein between protein A (SpA) and mutated firefly luciferase (Luc), was constructed. The fused gene was expressed in Escherichia coli and the resulting protein (SpA-LucTS) was purified with affinity chromatography. By changing a single amino acid, from Glu to Lys at the position 354 in the luciferase moiety, the thermostability of luciferase was improved. |
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| Keywords: | fusion protein luciferase point mutation protein A thermostability |
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