Degradation of Single Stranded Nucleic Acids by the
Chemical Nuclease Activity of the Metal Complex
[Cu(phen)(nal)]+
|
| |
Authors: | Norma Ramírez-Ramírez Guillermo Mendoza-Díaz Mario Pedraza-Reyes |
| |
Institution: | 1. Instituto de Investigación en Biología Experimental. Edificio “L”, Facultad de Química, Universidad de Guanajuato, Apartado Postal 187. Noria Alta S/N, Guanajuato, 36050 Guanajuato, Mexico.;2. Posgrado en Química Inorgánica, Facultad de Química, Universidad de Guanajuato, Apartado Postal 187, Guanajuato, 36050, Mexico, |
| |
Abstract: | The chemical design of metal complexes of the type Cu(phen)(antib)]+ (where antib is a quinolone or a
fluoroquinolone) has been carried out in an approach to better understand how the coordination of their
components affect the activity of quinolones. The ability of Cu(phen)(nal)]+ to interact with DNA in vivo
and its capacity to promote the degradation of plasmid and chromosomal DNA, under reductive conditions
has been previously reported. However whether this compound utilizes other intracellular targets to promote
bacterial killing was a question that deserved to be answered. In this paper, the studies of the chemical
nuclease properties encoded by the metal complex Cu(phen)(nal)]+ were extended by using different types of
single chain nucleic acids, i.e, ribosomal and tumor mosaic virus RNAs as well as poly-dA-dT. Our results
showed that degradation of the nucleic acids occurred only under reductive conditions. Although MPA and
3-mercaptoethanol were the chemical reducers that best assisted the nuclease reaction, other biological
compounds such as citric and succinic acid also were shown to act like reducers in that reaction. All.hough
the nuclease activity of Cu(phen)(nal)]+ was comparable to that exhibited by bis copper phenanthroline
Cu(phen)z]2+our results showed that none of the individual components of Cu(phen)(nal)]+ was able to
promote the degradation of either the RNAs or poly(dA-dT). These results strongly support the hypothesis
that the metal complex Cu(phen)(nal)] uses not only DNA but also RNA as targets to promote bacterial
killing. |
| |
Keywords: | |
|
|