影响杏黄兜兰种子萌发的因素

通过在不同条件下杏黄兜兰 (Paphiopedilumarmeniacum)种子萌发的观察 ,对影响其萌发的诸因子报道如下 :1)果实的生长期与种子的萌发率有关。实验表明种子采自生长期为 6 0d的果实 ,发芽率为 3 5 % ,采自 12 0d的果实 ,发芽率为 4 0 % ,采自 180d的果实 ,发芽率为 18 3%。 2 )培养基也会影响到种子的萌发 ,种子在 1 5MS内培养 ,萌发率明显高于培养于MS ,RE和改良HyponexNo 1内的种子。 3)培养基 (1 5MS)掺入添加物也会影响到种子的萌发率 ,如掺入 10 %椰子水会促使种子的萌发率达到很高的水平 ,掺入马铃薯泥 (5 0g L)或胰化胨 (2g L) ,种子的萌发率会达到较高的水平 ,而掺入香蕉泥则会对种子的萌发率产生负面影响 ,掺入活性炭 (2g L)会促使种子萌发和幼苗发育。 4 )与固态培养基相比 ,种子在液体悬浮培养基内的萌发速度更快 ,幼苗更为整齐划一。

Factors Affecting the Germination of Paphiopedilum armeniacum

Through our investigation of the seed germination of Paphiopedilum armeniacum under different conditions,the factors of direct effect on the seed germination are reported as follows:Growth phase of the fruit after pollination of the flower is related to germinative capacity of the seeds.The result demonstrated that germinative capacity of the seeds from the fruit with a growth phase of 60 days reached 3.5%,with a growth phase of 120 days reached 40%,with a growth phase of 180 days reached 18.3%.In the course of the seed germination,the culture medium will exert influence on the germinative capacity of the seeds.When the seeds were cultivated in the culture medium of 1/5MS,the germinative capacity is obviously higher than that cultivated in MS,RE and modified Hyponex No.1.If the culture medium (1/5MS) mixed with some additives,the germinative capacity of the seeds will show a change.When the culture medium mixed with 10% coconut milk,the germinative capacity of the seeds was promoted to the highest level,mixed with potato homogenate (50?g/L) or tryptone (2?g/L),it was promoted to a higher level,whereas mixed with banana homogenate,it was led to a side effect,mixed with activated charcoal (2?g/L) will improve the development in both seed germination and young plantlets.In comparison to traditional solid culture medium,the seeds in liquid suspension culture medium germinate faster and the plantlets will become more uniform.