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Activation of Peroxidase-Catalyzed Oxidation of Aromatic Amines with 2-Aminothiazole and Melamine
Authors:E I Karasyova  I V Naumchik  D I Metelitza
Institution:(1) National Academy of Sciences of Belarus, ul. Kuprevicha 5/2, Institute of Bioorganic Chemistry, Minsk, 220141, Belarus
Abstract:The peroxidase-catalyzed oxidation of 3,3",5,5"-tetramethylbenzidine (TMB), ortho-phenylenediamine (PDA), and 5-aminosalicylic acid (5-ASA) is significantly accelerated in the presence of 2-aminothiazole (AT) and melamine (MA), and an increase in their concentrations is associated with a parallel increase in the k cat and K m values for TMB and PDA. The activation of the peroxidase-catalyzed oxidation of TMB and PDA is quantitatively characterized by a coefficient (degree) agr (M–1) which significantly depends on pH in the range 6.2-6.4, 6.4-7.4, and 6.0-7.4 for the TMB–AT, TMB–MA, and PDA–MA pairs, respectively. An increase in the coefficient agr with increase in pH confirms nucleophilicity of activation of the peroxidase-catalyzed oxidation of the aromatic amines in the presence of AT and MA. Under optimal conditions the coefficients agr for the TMB–AT, PDA–AT, TMB–MA, and PDA–MA pairs vary in the limits of (1.90-3.53)·103 M–1.
Keywords:horseradish peroxidase  tetramethylbenzidine  ortho-phenylenediamine  5-aminosalicylic acid  activation of oxidation  2-aminothiazole  melamine  nucleophilic catalysis
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