Utilization of marker-assisted congenics to map two blood pressure quantitative trait loci in Dahl rats |
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Authors: | Alan Y Deng Julie Dutil Zsuzsa Sivo |
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Institution: | Research Centre-CHUM, H?tel Dieu, 7-132 Pavillon Jeanne Mance, 3840 rue St. Urbain, Montréal, Québec, H2W 1T8, Canada, CA
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Abstract: | A broad Chromosome (Chr) 10 region of the Dahl salt-sensitive (S) rat was shown by linkage and the use of congenic strains
to contain a blood pressure (BP) quantitative trait locus (QTL). To further narrow down the region harboring the QTL, four
congenic strains carrying smaller segments were made by replacing various segments of the S rats with the homologous segments
of the Lewis (LEW) rats. The construction of these congenic strains was facilitated by a genome-wide marker screening. One
congenic strain, assigned as S.L4, showed a BP-lowering effect, and the region harboring a BP QTL, designated QTL1, is localized
to a segment of about 15 cM. Two other strains, assigned as S.L2 and S.L5, contained an overlapping segment, and both showed
a BP-lowering effect. In contrast, the fourth congenic strain, assigned as S.L1, contained a smaller and shared fragment with
S.L2 and S.L5, but it did not have a BP-lowering effect. Deducing from the segment in common in S.L2 and S.L5, and not shared
between S.L1 and both congenic strains S.L2 and S.L5, the region harboring a QTL, designated as QTL2, was narrowed to about
12 cM. The current work showed the general applicability of the `speed congenic' approach to map and fine-map BP QTL.
Received: 1 February 2001 / Accepted: 29 March 2001 |
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