| Affiliation: | (1) Swiss Federal Research Station for Agroecology and Agriculture (FAL) Reckenholzstrasse 191, 8046 Zürich, Switzerland,;(2) Institute of Plant Biology, University of Zürich Zollikerstrasse 107, 8008 Zürich, Switzerland,;(3) Pharmaceutical Institute, University of Basel Benkenstrasse 254, 4108 Witterswil, Switzerland,;(4) Institut National de la Recherche Agronomique (INRA) Domaine de Crouelle, 234 rue du Brezet, 63039 Clermont-Ferrand, France,;(5) Indo-Swiss Collaboration in Biotechnology (ISCB), Institute of Biotechnology, ETH-Hoenggerberg, 8093 Zürich, Switzerland, |
| Abstract: | We constructed a genetic linkage map based on a cross between two Swiss winter wheat (Triticum aestivum L.) varieties, Arina and Forno. Two-hundred and forty F5 single-seed descent (SSD)-derived lines were analysed with 112 restriction fragment length polymorphism (RFLP) anonymous probes, 18 wheat cDNA clones coding for putative stress or defence-related proteins and 179 simple-sequence repeat (SSR) primer-pairs. The 309 markers revealed 396 segregating loci. Linkage analysis defined 27 linkage groups that could all be assigned to chromosomes or chromosome arms. The resulting genetic map comprises 380 loci and spans 3,086 cM with 1,131 cM for the A genome, 920 cM for the B genome and 1,036 cM for the D genome. Seventeen percent of the loci showed a significant (P < 0.05) deviation from a 1:1 ratio, most of them in favour of the Arina alleles. This map enabled the mapping of QTLs for resistance against several fungal diseases such as Stagonospora glume blotch, leaf rust and Fusarium head blight. It will also be very useful for wheat genetic mapping, as it combines RFLP and SSR markers that were previously located on separate maps. S. Paillard and T. Schnurbusch contributed equally to the work |
