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Rhinosporidium seeberi: Light,phase contrast,fluorescent and scanning electron microscopic study
Authors:J. S. Moses  C. Balachandran  Bejan Singh  A. Shanmugan  Dinakar Moses
Affiliation:(1) Madras Veterinary College, Madras-7;(2) Veterinary College and Research Institute, 637 002 Namakkal;(3) C.S.I. Hospital Neyoor, India;(4) Government Head Quarters Hospital, Nagercoil;(5) Madras, India
Abstract:Phase contrast microscopic study indicated the multilayered structure of the sporangial wall of R. seeberi while the scanning electronmicroscopic study revealed a trilaminated wall compared to a thick double walled light microscopic structure. The scanning electronmicroscopy revealed the spores of varying sizes which were found either discretely or in groups interconnected and seen attached to the inner aspect of the sporangial wall. Autofluorescence of sporangia and spores was observed under microscope. Acridine orange staining revealed the presence of DNA materials in the spore and sporangia.
Keywords:Rhinosporidium seeberi  phase contrast  fluorescence  scanning electronmicroscopy
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