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Enzymatic properties of mitochondria isolated from normal and vitamin B12-deficient rats. |
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| Authors: | M A Matlib E P Frenkel A Mukherjee J Henslee P A Srere |
| Institution: | 1. Pre-Clinical Sciences Unit and Nuclear Medicine Service, Veterans Administration Medical Center, Dallas, Texas 75216 U.S.A.;2. The Department of Biochemistry and the Evelyn L. Overton Hematology-Oncology Research Laboratory Dallas, Texas 75235 U.S.A.;3. Department of Internal Medicine, The University of Texas Health Science Center, Dallas, Texas 75235 U.S.A. |
| Abstract: | The mitochondria from livers of normal and vitamin B12-deprived animals were compared. The rate of oxygen uptake (states 3 and 4) of mitochondria from vitamin B12-deprived livers was higher than that of normal mitochondria while the respiratory control index of B12-deprived mitochondria was lower than normal. Most Krebs cycle enzyme activities were increased in the hepatic mitochondria from B12-deprived animals. Two-dimensional gel electrophoresis of mitochondrial matrix proteins also indicated that an increase in Krebs cycle enzyme quantities occurred but the increase was not general for all mitochondrial proteins. |
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