Ethylcarbamoyl protection for cysteine in the preparation of peptide-conjugate immunogens. |
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Authors: | J Blake B A Woodworth L Litzi-Davis W L Cosand |
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Institution: | Bristol-Myers Squibb, Seattle, WA. |
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Abstract: | During the solid-phase synthesis of over 100 peptides, we have observed that the ethylcarbamoyl group is useful for the side chain protection of cysteine in peptides containing a single cysteine residue. The ethylcarbamoyl group is stable to the conditions of acidolytic cleavage, purification and long term storage. Brief treatment of peptides containing an S-ethylcarbamoyl-cysteine residue with aqueous sodium hydroxide gives the deprotected cysteine peptide that can be coupled to carrier molecules such as proteins to give immunogen conjugates. |
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