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Molecular characterization and evaluation of pectinase and cellulase production of Penicillium spp.
Authors:Jorge Fernando Pereira  Marisa Vieira de Queiroz  Eliane Aparecida Gomes  Júpiter Israel Muro-Abad  Elza Fernandes de Araújo
Affiliation:(1) Departamento de Microbiologia/BIOAGRO, Universidade Federal de Viçosa, Viçosa-MG, Brazil;(2) Present address: EMBRAPA Milho e Sorgo, 35701-970 Sete Lagoas-MG, Brazil
Abstract:Penicillium species were analyzed with molecular markers and for pectinase and cellulase production. RAPD and PCR-RFLP analysis indicated high polymorphism among at least 5 of 10 Penicillium species. Five species were chosen for pectinase and cellulase production in liquid medium and four of which appeared similar based on molecular analyses. P. brevicompactum and P. griseoroseum gave the highest pectinase production and were highly divergent by molecular techniques.
Keywords:cellulase  pectin lyase  Penicillium  polygalacturonase  RAPD  rDNA
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