| 人甲状旁腺激素在大肠杆菌中的表达及鉴定 |
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| 引用本文： | 方宏清 戴红梅 李彦英 赵洪亮 刘志敏 朱厚础 马清钧 陈惠鹏. 人甲状旁腺激素在大肠杆菌中的表达及鉴定[J]. 生物工程学报, 2003, 19(1): 102-106 |
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| 作者姓名： | 方宏清 戴红梅 李彦英 赵洪亮 刘志敏 朱厚础 马清钧 陈惠鹏 |
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| 作者单位： | 北京生物工程研究所,北京,100071 |
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| 基金项目： | 军事医学科学院开发基金，国家 863项目资助 (No .2 0 0 1AA2 15 45 1 3 )~~ |
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| 摘 要： | 化学合成人甲状旁腺激素（hPTH）全长基因，克隆到大肠杆菌表达载体pBV220和pET22b中，获得了高表达。经破菌、阳离子交换层析、反相层析纯化获得了纯度大于95%的纯品。N端测序、质谱分析结果表明重组hPTH 结果完整，N端无Met或fMet。生物活性试验证明重组hPTH具有激活腺苷酸环化酶、增加骨质量和骨密度等作用。
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| 关 键 词： | 人甲状旁腺激素， 大肠杆菌， 基因表达 |
| 文章编号： | 1000-3061(2003)01-0102-05 |
| 修稿时间： | 2002-07-19 |
High Expression and Characterization of Human Parathyroid Hormone in Escherichia coli |
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| Hong-Qing Fang,Hong-Mei Dai,Yan-Ying Li,Hong-Liang Zhao,Bing-Bing Deng,Chong Xue,Zhi-Min Liu,Hou-Chu Zhu,Qing-Jun Ma,Hui-Peng Chen. High Expression and Characterization of Human Parathyroid Hormone in Escherichia coli[J]. Chinese journal of biotechnology, 2003, 19(1): 102-106 |
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| Authors: | Hong-Qing Fang Hong-Mei Dai Yan-Ying Li Hong-Liang Zhao Bing-Bing Deng Chong Xue Zhi-Min Liu Hou-Chu Zhu Qing-Jun Ma Hui-Peng Chen |
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| Affiliation: | Beijing Institute of Biotechnology, Beijing 100071, China. fanghongqing@hotmail.com |
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| Abstract: | Human parathyroid hormone(hPTH) was highly expressed in Escherichia coli by inserted the synthesized whole hPTH cDNA into the vectors pBV220 and pET22b.After expression and disruption,the purified product was acquired through cation exchange chromatography and reverse phase chromatography.From the results of N terminal sequencing and MALDI TOF MS analysis the recombiant prtein was indentified as intact hPTH.In in vitro Bioassays the recombinant hPTH stimulated adenylate cyclase as the standard did. In ovariectomized rats the recombinant hPTH markedly increased the femoral bone mass and bone mineral density. |
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| Keywords: | human parathyroid hormone Escherichia coli gene expression |
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