Structure and organization of the 5S rRNA genes (5S DNA) inPinus radiata (Pinaceae) |
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| Authors: | Susan Wilkie Gorman Robert Dixon Teasdale Christopher Ashley Cullis |
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| Affiliation: | (1) Case Western Reserve University, 10900 Euclid Avenue, 44106-7068 Cleveland, Ohio, USA;(2) Southwestern medical Center at Dallas, University of Texas, 5323 Harry Hines Blvd., 75235-9038 Dallas, Texas, USA;(3) Division of Science and Technology, Griffith University, 4105 Queensland, Australia |
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| Abstract: | A 5S rRNA gene (5S DNA) from the coniferPinus radiataD. Don has been cloned and characterized at the nucleotide, genomic and chromosomal levels. Sequencing revealed a repeat unit of 524 base pairs which is present in approximately 3000 copies per diploid genome. Two-dimensional gel electrophoresis indicated that these copies are organized in tandem arrays of various length. Using in situ hybridization techniques, the tandem arrays appear to be present on all of the chromosomes. This complexity of chromosomal organization contrasts markedly with the few sites of uniform length found in angiosperm plants such as wheat, pea, and maize. |
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| Keywords: | Gymnosperms Pinaceae Pinus Dispersed repeats 5S rRNA two-dimensional gel electrophoresis in situ hybridizations |
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